Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons

Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons

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Adult dorsal root ganglia neurons are among the few adult neuronal cell read more types that can be purified and grown relatively easily in dissociated cell culture.Here we describe a procedure for the isolation and growth of dissociated adult mouse merlin wizard costume DRG neurons using Percoll gradients and a chemically defined medium.These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function.